Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria.

ABSTRACT: Native cell-free transcription-translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host, Bacillus megaterium, is a giant Gram-positive bacterium with potential biotechnology applications, although many of its regulatory elements remain uncharacterized. Here, we have developed a rapid automated platform for measuring and modeling in vitro cell-free reactions and have applied this to B. megaterium to quantify a range of ribosome binding site variants and previously uncharacterized endogenous constitutive and inducible promoters. To provide quantitative models for cell-free systems, we

SUBMITTER: Moore SJ 

PROVIDER: S-EPMC5948957 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature