An atypical N-ethylmaleimide sensitive factor enables the viability of nematode-resistant Rhg1 soybeans.

ABSTRACT: N-ethylmaleimide sensitive factor (NSF) and α-soluble NSF attachment protein (α-SNAP) are essential eukaryotic housekeeping proteins that cooperatively function to sustain vesicular trafficking. The "resistance to Heterodera glycines 1" (Rhg1) locus of soybean (Glycine max) confers resistance to soybean cyst nematode, a highly damaging soybean pest. Rhg1 loci encode repeat copies of atypical α-SNAP proteins that are defective in promoting NSF function and are cytotoxic in certain contexts. Here, we discovered an unusual NSF allele (Rhg1-associated NSF on chromosome 07; NSF_RAN07 ) in Rhg1⁺ germplasm. NSF_RAN07 protein modeling to mammalian NSF/α-SNAP complex structures indicated that at least thre

SUBMITTER: Bayless AM 

PROVIDER: S-EPMC5948960 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature