High-quality human preimplantation embryos actively influence endometrial stromal cell migration.

ABSTRACT:

Purpose

The purpose of this paper is to study whether human preimplantation embryos regulate endometrial stromal cell (hESC) migration.

Methods

Primary hESCs were isolated from fertile patients undergoing hysterectomy for benign conditions (uterine scar niche n = 3, dysmenorrhea n = 2; no hormonal treatment). Migration and proliferation assays were performed by culturing decidualized or non-decidualized hESCs in the presence of embryo conditioned medium (ECM) from high-quality embryos (fragmentation ≤ 20%) or from low-quality embryos (fragmentation > 20%) or in non-conditioned medium from the same dishes (control). ECM samples from 425 individually cultured human embryos were used in this study.

Results

ECM from high-quality embryos, i.e., with a low percentage of fra

SUBMITTER: Berkhout RP 

PROVIDER: S-EPMC5949101 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature