Phage display selected magnetite interacting Adhirons for shape controlled nanoparticle synthesis.

ABSTRACT: Adhirons are robust, well expressing, peptide display scaffold proteins, developed as an effective alternative to traditional antibody binding proteins for highly specific molecular recognition applications. This paper reports for the first time the use of these versatile proteins for material binding, and as tools for controlling material synthesis on the nanoscale. A phage library of Adhirons, each displaying two variable binding loops, was screened to identify specific proteins able to interact with [100] faces of cubic magnetite nanoparticles. The selected variable regions display a strong preference for basic residues such as lysine. Molecular dynamics simulations of amino acid adsorption onto a [100] magnetite surface provides a rationale for these interactions, with the lowest adsor

SUBMITTER: Rawlings AE 

PROVIDER: S-EPMC5949846 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature