In vivo demonstration of an active tumor pretargeting approach with peptide nucleic acid bioconjugates as complementary system.

ABSTRACT: A novel, promising strategy for cancer diagnosis and therapy is the use of a pretargeting approach. For this purpose, the non-natural DNA/RNA analogues Peptide Nucleic Acids (PNAs) are ideal candidates as in vivo recognition units due to their high metabolic stability and lack of unspecific accumulation. In the pretargeting approach, an unlabeled, highly specific antibody-PNA conjugate has sufficient time to target a tumor before administration of a small fast-clearing radiolabeled complementary PNA that hybridizes with the antibody-PNA conjugate at the tumor site. Herein, we report the first successful application of this multistep process using a PNA-modified epidermal growth factor receptor (EGFR) specific antibody (cetuximab) and a complementary ^99mTc-labeled PNA.

SUBMITTER: Leonidova A 

PROVIDER: S-EPMC5949856 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature