Role of quantitative p16^INK4A mRNA assay and digital reading of p16^INK4A immunostained sections in diagnosis of cervical intraepithelial neoplasia.

ABSTRACT: Visual interpretation of cervical biopsies is subjective and variable, generally showing fair to moderate inter-reader agreement in distinguishing high from low grade cervical intraepithelial neoplasia (CIN). We investigated the performance of two objective p16 quantitative tests in comparison with visual assessment: (i) p16-mRNA assay and (ii) digital analysis of sections stained for p16 protein. The primary analysis considered 232 high-risk human papilloma virus positive (HPV+) samples from diagnostic cervical specimens. A p16 RT-qPCR (p16-mRNA assay) was run on mRNA extracted from formalin-fixed paraffin-embedded sections. Two p16 immunohistochemistry (IHC) readings, a visual read by a histopathologist (Visual IHC) and a digital read of a high-resolution scan (Digital IHC), were done on

SUBMITTER: Vasiljevic N 

PROVIDER: S-EPMC5949861 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature