A cassette of basic amino acids in histone H2B regulates nucleosome dynamics and access to DNA damage.

ABSTRACT: Nucleosome dynamics, such as spontaneous DNA unwrapping, are postulated to have a critical role in regulating the access of DNA repair machinery to DNA lesions within nucleosomes. However, the specific histone domains that regulate nucleosome dynamics and the impact of such changes in intrinsic nucleosome dynamics on DNA repair are not well understood. Previous studies identified a highly conserved region in the N-terminal tail of histone H2B known as the histone H2Brepression (or HBR) domain, which has a significant influence on gene expression, chromatin assembly, and DNA damage formation and repair. However, the molecular mechanism(s) that may account for these observations are limited. In this study, we characterized the stability and dynamics of ΔHBR mutant nucleo

SUBMITTER: Rodriguez Y 

PROVIDER: S-EPMC5949990 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature