WWC3 inhibits epithelial-mesenchymal transition of lung cancer by activating Hippo-YAP signaling.

ABSTRACT:

Background

Though we recently reported that the WWC3 inhibits the invasiveness and metastasis of lung cancer by activating the Hippo pathway, the impact and underlying mechanisms of this process still remain unclear.

Methods

To identify the role of WWC3 in epithelial-mesenchymal transition of lung cancer, we performed immunohistochemistry to detect the expression levels of WWC3 and EMT-related biomarker, and analyzed their correlations in a cohort of 127 patients with NSCLC. Wound healing assay and cell invasion assay were applied to explore cell invasive ability change after WWC3 knockdown. qRT-PCR and immunoblotting were performed to assess mRNA and protein levels of EMT-related biomarkers and the main molecules changes of Hippo signaling caused by WWC3. Immunoprecipition

SUBMITTER: Han Q 

PROVIDER: S-EPMC5951220 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature