The SMAD2/3 interactome reveals that TGFβ controls m⁶A mRNA methylation in pluripotency.

ABSTRACT: The TGFβ pathway has essential roles in embryonic development, organ homeostasis, tissue repair and disease. These diverse effects are mediated through the intracellular effectors SMAD2 and SMAD3 (hereafter SMAD2/3), whose canonical function is to control the activity of target genes by interacting with transcriptional regulators. Therefore, a complete description of the factors that interact with SMAD2/3 in a given cell type would have broad implications for many areas of cell biology. Here we describe the interactome of SMAD2/3 in human pluripotent stem cells. This analysis reveals that SMAD2/3 is involved in multiple molecular processes in addition to its role in transcription. In particular, we identify a functional interaction with the METTL3-METTL14-WTAP complex, which mediates the c

SUBMITTER: Bertero A 

PROVIDER: S-EPMC5951268 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature