Characterization of a new apple luteovirus identified by high-throughput sequencing.

ABSTRACT:

Background

'Rapid Apple Decline' (RAD) is a newly emerging problem of young, dwarf apple trees in the Northeastern USA. The affected trees show trunk necrosis, cracking and canker before collapse in summer. In this study, we discovered and characterized a new luteovirus from apple trees in RAD-affected orchards using high-throughput sequencing (HTS) technology and subsequent Sanger sequencing.

Methods

Illumina NextSeq sequencing was applied to total RNAs prepared from three diseased apple trees. Sequence reads were de novo assembled, and contigs were annotated by BLASTx. RT-PCR and 5'/3' RACE sequencing were used to obtain the complete genome of a new virus. RT-PCR was used to detect the virus.

Results

Three common apple viruses and a new luteovirus were identified fr

SUBMITTER: Liu H 

PROVIDER: S-EPMC5952423 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature