Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose.

ABSTRACT:

Background

Both industrial biotechnology and the use of cellulosic biomass as feedstock for the manufacture of various commercial goods are prominent features of the bioeconomy. In previous work, with the aim of developing a consolidated bioprocess for cellulose bioconversion, we conferred cellulolytic activity of Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species. However, further engineering this strain often leads to the loss of previously introduced heterologous genes due to the presence of multiple LoxP sites when using Cre-recombinase to remove previously employed selection markers.

Results

In the present study, we first optimized the strategy of expression of multiple cellulases and rescued selection mak

SUBMITTER: Guo ZP 

PROVIDER: S-EPMC5952637 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature