Transcriptome analysis of alcohol-treated microglia reveals downregulation of beta amyloid phagocytosis.

ABSTRACT:

Background

Microglial activation contributes to the neuropathology associated with chronic alcohol exposure and withdrawal, including the expression of inflammatory and anti-inflammatory genes. In the current study, we examined the transcriptome of primary rat microglial cells following incubation with alcohol alone, or alcohol together with a robust inflammatory stimulus.

Methods

Primary microglia were prepared from mixed rat glial cultures. Cells were incubated with 75 mM ethanol alone or with proinflammatory cytokines ("TII": IL1β, IFNγ, and TNFα). Isolated mRNA was used for RNAseq analysis and qPCR. Effects of alcohol on phagocytosis were determined by uptake of oligomeric amyloid beta.

Results

Alcohol induced nitrite production in control cells and increased nitr

SUBMITTER: Kalinin S 

PROVIDER: S-EPMC5952855 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature