Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells.

ABSTRACT:

Aims

High levels of glucose and reactive carbonyl intermediates of its degradation pathway such as methylglyoxal (MG) may contribute to diabetic complications partly via increased generation of reactive oxygen species (ROS). This study focused on glutathione peroxidase-1 (GPx1) expression and the impact of carbonylation as an oxidative protein modification on GPx1 abundance and activity in human umbilical vein endothelial cells (HUVEC) under conditions of mild to moderate oxidative stress.

Results

High extracellular glucose and MG enhanced intracellular ROS formation in HUVECs. Protein carbonylation was only transiently augmented pointing to an effective antioxidant defense in these cells. Nitric oxide synthase expression was decreased under hyperglycemic conditions but incr

SUBMITTER: Sultan CS 

PROVIDER: S-EPMC5952877 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature