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GC Content of Early Metazoan Genes and Its Impact on Gene Expression Levels in Mammalian Cell Lines.

ABSTRACT: With the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, a mammalian cell line is a generally used strategy in structure-function analysis. However, this might turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show its impact on gene expression levels in human cell lines, and, importantly, how this expression efficiency can be improved. Optimization of the GC3 content in the coding sequences of cadherin, alpha-catenin, and paracaspase of Trichoplax adhaerens dramatically increased the expression of these basal animal genes in human cell lines.

SUBMITTER: Gul IS 

PROVIDER: S-EPMC5952964 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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GC Content of Early Metazoan Genes and Its Impact on Gene Expression Levels in Mammalian Cell Lines.

Gul Ismail Sahin IS   Staal Jens J   Hulpiau Paco P   De Keuckelaere Evi E   Kamm Kai K   Deroo Tom T   Sanders Ellen E   Staes Katrien K   Driege Yasmine Y   Saeys Yvan Y   Beyaert Rudi R   Technau Ulrich U   Schierwater Bernd B   van Roy Frans F  

Genome biology and evolution 20180301 3

With the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, a mammalian cell line is a generally used strategy in structure-function analysis. However, this might turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show it  ...[more]

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