A ratiometric two-photon probe for quantitative imaging of mitochondrial pH values.

ABSTRACT: Mitochondrial pH (pH_mito) is known to be alkaline (near 8.0) and has emerged as a potential factor for mitochondrial function and disorder. We have developed a ratiometric two-photon probe (CMP1) for quantitative analysis of pH_mito in live cells and tissues. This probe is designed to function by controlling the intramolecular charge transfer from 2-naphthol, having an ideal pK_a value (7.86 ± 0.05) in the cells to monitor pH_mito. This transition results in a marked yellow to red emission color change in response to pH alterations from 6.0 to 9.0. CMP1 exhibits easy loading, selective and robust staining ability of mitochondria, low cytotoxicity, and bright two-photon excited fluorescence in situ, thereby allowing quanti

SUBMITTER: Sarkar AR 

PROVIDER: S-EPMC5953010 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature