Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array.

ABSTRACT: The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral information in a single data acquisition, however, instrumentation for HSI is typically complex, bulky and expensive. We sought to overcome these limitations using a novel robust and low cost HSI camera based on a spectrally resolved detector array (SRDA). We integrated this HSI camera into a wide-field reflectance-based imaging system operating in the near-infrared range to assess the suitability for in vivo imaging of exogenous fluorescent contrast agents. Using this fluore

SUBMITTER: Luthman AS 

PROVIDER: S-EPMC5953275 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature