Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12.

ABSTRACT: The substrate profiles for three uncharacterized enzymes (YcjM, YcjT, and YcjU) that are expressed from a cluster of 12 genes ( ycjM-W and ompG) of unknown function in Escherichia coli K-12 were determined. Through a comprehensive bioinformatic and steady-state kinetic analysis, the catalytic function of YcjT was determined to be kojibiose phosphorylase. In the presence of saturating phosphate and kojibiose (α-(1,2)-d-glucose-d-glucose), this enzyme catalyzes the formation of d-glucose and β-d-glucose-1-phosphate ( k_cat = 1.1 s^-1, K_m = 1.05 mM, and k_cat/ K_m = 1.12 × 10³ M^-1 s^-1). Additionally, it was also shown that in the presence of β-d-glucose-1-phosphate, YcjT can catalyze the formation of other dis

SUBMITTER: Mukherjee K 

PROVIDER: S-EPMC5953851 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature