Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana.

ABSTRACT: Alternative splicing, in which one gene produce multiple transcripts, may influence how adaptive genes respond to specific environments. A newly produced transcriptome of Drosophila montana shows the Gs1-like (Gs1l) gene to express multiple splice variants and to be down regulated in cold acclimated flies with increased cold tolerance. Gs1l's effect on cold tolerance was further tested by injecting cold acclimated and non-acclimated flies from two distantly located northern and southern fly populations with double stranded RNA (dsRNA) targeting Gs1l. While both populations had similar cold acclimation responses, dsRNA injections only effected the northern population. The nature of splicing expression was then investigated in the northern population by confirming which Gs1l variants are pre

SUBMITTER: Hopkins D 

PROVIDER: S-EPMC5953924 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature