Generation and characterization of a novel recombinant scFv antibody specific for Campylobacter jejuni.

ABSTRACT: Campylobacter jejuni is a leading cause of foodborne illness worldwide, mainly due to consumption and handling of contaminated raw chicken. Rapid detection methods for C. jejuni are vital for monitoring contamination levels in chicken products and reducing human Campylobacteriosis cases. The 'gold standard' culture-based method of Campylobacter detection takes 3-5 days and is too slow to permit effective intervention. Immuno-based methods are faster, but usually necessitate use of animals or hybridoma technology to produce antibodies; making them difficult and expensive to produce. Here, we report the generation and characterization of recombinant single-chain variable fragment (scFv) antibodies specific for C. jejuni cells, and evaluation of one scFv antibody for an immunomagnetic separat

SUBMITTER: Nzuma RM 

PROVIDER: S-EPMC5953994 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature