Assessment of the scalability of a microtiter plate system for screening of oleaginous microorganisms.

ABSTRACT: Recent developments in molecular biology and metabolic engineering have resulted in a large increase in the number of strains that need to be tested, positioning high-throughput screening of microorganisms as an important step in bioprocess development. Scalability is crucial for performing reliable screening of microorganisms. Most of the scalability studies from microplate screening systems to controlled stirred-tank bioreactors have been performed so far with unicellular microorganisms. We have compared cultivation of industrially relevant oleaginous filamentous fungi and microalga in a Duetz-microtiter plate system to benchtop and pre-pilot bioreactors. Maximal glucose consumption rate, biomass concentration, lipid content of the biomass, biomass, and lipid yield values showed good sca

SUBMITTER: Kosa G 

PROVIDER: S-EPMC5954000 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature