Rapid genotype "independent" Zea mays L. (maize) transformation via direct somatic embryogenesis.

ABSTRACT: Constitutive expression of the Zea mays L. (maize) morphogenic transcription factors Baby Boom (Bbm) and Wuschel2 (Wus2) in maize can not only greatly increase transformation efficiency but can also induce phenotypic abnormalities and sterility. In an effort to alleviate the pleiotropic effects of constitutive expression, a genome wide search was undertaken to find suitable maize promoters to drive tissue and timing-specific expression of the transformation enhancing genes Bbm and Wus2. A promoter from a maize phospholipid transferase protein gene (Zm-PLTP_pro ) was identified based on its expression in leaves, embryos, and callus while being downregulated in roots, meristems, and reproductive tissues. When

SUBMITTER: Lowe K 

PROVIDER: S-EPMC5954046 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature