Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells.

ABSTRACT: The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes of undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma formation in vivo provide direct tests of differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions and under conditions promoting differentiation to ectoderm, mesoderm, or endoderm lineages, are sufficient to assess the differentiation potential of PSCs. However, teratoma analysis by histologic examination and by TeratoScore, which estimates differential gene expression in each tumor, not only measures differentiation but also allows insight into a P

SUBMITTER: International Stem Cell Initiative 

PROVIDER: S-EPMC5954055 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature