Differential Activity of Voltage- and Ca²⁺-Dependent Potassium Channels in Leukemic T Cell Lines: Jurkat Cells Represent an Exceptional Case.

ABSTRACT: Activation of resting T cells relies on sustained Ca²⁺ influx across the plasma membrane, which in turn depends on the functional expression of potassium channels, whose activity repolarizes the membrane potential. Depending on the T-cells subset, upon activation the expression of Ca²⁺- or voltage-activated K⁺ channels, KCa or Kv, is up-regulated. In this study, by means of patch-clamp technique in the whole cell mode, we have studied in detail the characteristics of Kv and KCa currents in resting and activated human T cells, the only well explored human T-leukemic cell line Jurkat, and two additional human leukemic T cell lines, CEM and MOLT-3. Voltage dependence of activation and inactivation of Kv1.3 current were shifted up to by 15 mV to more negative p

SUBMITTER: Valle-Reyes S 

PROVIDER: S-EPMC5954129 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature