Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy.

ABSTRACT: Correction of chromatic shift is necessary for precise registration of multicolor fluorescence images of biological specimens. New emerging technologies in fluorescence microscopy with increasing spatial resolution and penetration depth have prompted the need for more accurate methods to correct chromatic aberration. However, the amount of chromatic shift of the region of interest in biological samples often deviates from the theoretical prediction because of unknown dispersion in the biological samples. To measure and correct chromatic shift in biological samples, we developed a quadrisection phase correlation approach to computationally calculate translation, rotation, and magnification from reference images. Furthermore, to account for local chromatic shifts, images are split into small

SUBMITTER: Matsuda A 

PROVIDER: S-EPMC5954143 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature