Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes.

ABSTRACT: Active diffusion of intracellular components is emerging as an important process in cell biology. This process is mediated by complex assemblies of molecular motors and cytoskeletal filaments that drive force generation in the cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have been precisely characterized in vitro by single molecule approaches, but their in vivo behavior remains elusive. Here, we study the active diffusion of vesicles in mouse oocytes, where this process plays a key role in nuclear positioning during development, and combine an experimental and theoretical framework to extract molecular-scale force kinetics (force, power stroke, and velocity) of the in vivo active process. Assuming a single dominant process, we find that the nonequilibrium acti

SUBMITTER: Ahmed WW 

PROVIDER: S-EPMC5954280 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature