Effects of mRNA Degradation and Site-Specific Transcriptional Pausing on Protein Expression Noise.

ABSTRACT: Genetically identical cells exhibit diverse phenotypes even when experiencing the same environment. This phenomenon in part originates from cell-to-cell variability (noise) in protein expression. Although various kinetic schemes of stochastic transcription initiation are known to affect gene expression noise, how posttranscription initiation events contribute to noise at the protein level remains incompletely understood. To address this question, we developed a stochastic simulation-based model of bacterial gene expression that integrates well-known dependencies between transcription initiation, transcription elongation dynamics, mRNA degradation, and translation. We identified realistic conditions under which mRNA lifetime and transcriptional pauses modulate the protein expression noise i

SUBMITTER: Kim S 

PROVIDER: S-EPMC5954620 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature