Base pairing involving artificial bases in vitro and in vivo.

ABSTRACT: Herein we report the synthesis of N⁸-glycosylated 8-aza-deoxyguanosine (N⁸-8-aza-dG) and 8-aza-9-deaza-deoxyguanosine (N⁸-8-aza-9-deaza-dG) nucleotides and their base pairing properties with 5-methyl-isocytosine (d-isoC^Me), 8-amino-deoxyinosine (8-NH₂-dI), 1-N-methyl-8-amino-deoxyinosine (1-Me-8-NH₂-dI), 7,8-dihydro-8-oxo-deoxyinosine (8-Oxo-dI), 7,8-dihydro-8-oxo-deoxyadenosine (8-Oxo-dA), and 7,8-dihydro-8-oxo-deoxyguanosine (8-Oxo-dG), in comparison with the d-isoC^Me:d-isoG artificial genetic system. As demonstrated by T_m measurements, the N⁸-8-aza-dG:d-isoC^Me base pair formed less stable duplexes as the C:G and d-isoC^Me:d-iso

SUBMITTER: Bande O 

PROVIDER: S-EPMC5954848 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature