Single-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.

ABSTRACT: Direct lineage conversion offers a new strategy for tissue regeneration and disease modelling. Despite recent success in directly reprogramming fibroblasts into various cell types, the precise changes that occur as fibroblasts progressively convert to the target cell fates remain unclear. The inherent heterogeneity and asynchronous nature of the reprogramming process renders it difficult to study this process using bulk genomic techniques. Here we used single-cell RNA sequencing to overcome this limitation and analysed global transcriptome changes at early stages during the reprogramming of mouse fibroblasts into induced cardiomyocytes (iCMs). Using unsupervised dimensionality reduction and clustering algorithms, we identified molecularly distinct subpopulations of cells during reprogrammi

SUBMITTER: Liu Z 

PROVIDER: S-EPMC5954984 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature