A human monoclonal antibody that specifically binds and inhibits the staphylococcal complement inhibitor protein SCIN.

ABSTRACT: Staphylococcus aureus is a serious public health burden causing a wide variety of infections. Earlier detection of such infections could result in faster and more directed therapies that also prevent resistance development. Human monoclonal antibodies (humAbs) are promising tools for diagnosis and therapy owing to their relatively straightforward synthesis, long history of safe clinical use and high target specificity. Here we show that the humAb 6D4, which was obtained from a random screen of B-cells producing antibodies that bind to whole cells of S. aureus, targets the staphylococcal complement inhibitor (SCIN). The epitope recognized by 6D4 was localized to residues 26 to 36 in the N-terminus of SCIN, which overlap with the active site. Accordingly, 6D4 can inhibit SCIN activity as dem

SUBMITTER: Hoekstra H 

PROVIDER: S-EPMC5955450 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature