A D-Alanine auxotrophic live vaccine is effective against lethal infection caused by Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus infections are becoming a major global health issue due to the rapid emergence of multidrug-resistant strains. Therefore, there is an urgent need to develop an effective vaccine to prevent and control these infections. In order to develop a universal immunization strategy, we constructed a mutant derivative of S. aureus 132 which lacks the genes involved in D-alanine biosynthesis, a structural component of cell wall peptidoglycan. This unmarked deletion mutant requires the exogenous addition of D-alanine for in vitro growth. The aim of this study was to examine the ability of this D-alanine auxotroph to induce protective immunity against staphylococcal infection. Our findings demonstrate that this deletion mutant is highly attenuated, elicits a protective immune respo

SUBMITTER: Moscoso M 

PROVIDER: S-EPMC5955480 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature