Transcriptome analysis reveals a stress response of Shewanella oneidensis deprived of background levels of ionizing radiation.

ABSTRACT: Natural ionizing background radiation has exerted a constant pressure on organisms since the first forms of life appeared on Earth, so that cells have developed molecular mechanisms to avoid or repair damages caused directly by radiation or indirectly by radiation-induced reactive oxygen species (ROS). In the present study, we investigated the transcriptional effect of depriving Shewanella oneidensis cultures of background levels of radiation by growing the cells in a mine 655 m underground, thus reducing the dose rate from 72.1 to 0.9 nGy h-1 from control to treatment, respectively. RNASeq transcriptome analysis showed the differential expression of 4.6 and 7.6% of the S. oneidensis genome during early- and late-exponential phases of growth, respectively. The greatest change observed in t

SUBMITTER: Castillo H 

PROVIDER: S-EPMC5955497 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature