A guide to automated apoptosis detection: How to make sense of imaging flow cytometry data.

ABSTRACT: Imaging flow cytometry is a powerful experimental technique combining the strength of microscopy and flow cytometry to enable high-throughput characterization of cell populations on a detailed microscopic scale. This approach has an increasing importance for distinguishing between different cellular phenotypes such as proliferation, cell division and cell death. In the course of undergoing these different pathways, each cell is characterized by a high amount of properties. This makes it hard to filter the most relevant information for cell state discrimination. The traditional methods for cell state discrimination rely on dye based two-dimensional gating strategies ignoring information that is hidden in the high-dimensional property space. In order to make use of the information ignored by

SUBMITTER: Pischel D 

PROVIDER: S-EPMC5955558 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature