Comparison of Direct and Indirect cold atmospheric-pressure plasma methods in the B₁₆F₁₀ melanoma cancer cells treatment.

ABSTRACT: In this study a novel method was implemented and investigated in order to destroy cancer cells inside the mouse body on a clinical level. In the case of in-vitro study, MTT assay was employed to discover an effective dose of applied plasma and distinguish the plasma effect in direct and in indirect treatments. Tumor growth was also measured in in-vivo section so that the effectiveness of direct and indirect treatments could be compared. Furthermore, an investigation was conducted to study the interferences between a conventional method (chemotherapy) and plasma treatment so as to increase the effectiveness of treatment inside the body. Hematoxylin and Eosin, Flow Cytometry, TUNEL and Western Blot assay were used to investigate any cell alteration and the impact of various treatment methods

SUBMITTER: Saadati F 

PROVIDER: S-EPMC5955918 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature