FACS-Seq analysis of Pax3-derived cells identifies non-myogenic lineages in the embryonic forelimb.

ABSTRACT: Skeletal muscle in the forelimb develops during embryonic and fetal development and perinatally. While much is known regarding the molecules involved in forelimb myogenesis, little is known about the specific mechanisms and interactions. Migrating skeletal muscle precursor cells express Pax3 as they migrate into the forelimb from the dermomyotome. To compare gene expression profiles of the same cell population over time, we isolated lineage-traced Pax3⁺ cells (Pax3 ^EGFP ) from forelimbs at different embryonic days. We performed whole transcriptome profiling via RNA-Seq of Pax3⁺ cells to construct gene networks involved in different stages of embryonic and fetal development. With this, we identified genes involved in the skeletal, muscular, vascular, nervous

SUBMITTER: Singh AJ 

PROVIDER: S-EPMC5956100 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature