Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators.

ABSTRACT:

Background

Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance.

Results

Two sets of novel minimal transcriptional activators (DBD_ace2-VP16 and DBD_cre1-VP16) were designed and expressed in T. reesei RUT C30. Expression of DBD_ace2-VP16 and DBD_cre1-VP16 improved cellulase production under induction (avicel or lactose) and repression (glucose) conditions, respective

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5956553 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature