A method for estimating relative changes in the synaptic density in Drosophila central nervous system.

ABSTRACT: BACKGROUND:Synapse density is an essential indicator of development and functioning of the central nervous system. It is estimated indirectly through the accumulation of pre and postsynaptic proteins in tissue sections. 3D reconstruction of the electron microscopic images in serial sections is one of the most definitive means of estimating the formation of active synapses in the brain. It is tedious and highly skill-dependent. Confocal imaging of whole mounts or thick sections of the brain provides a natural alternative for rapid gross estimation of the synapse density in large areas. The optical resolution and other deep-tissue imaging aberrations limit the quantitative scope of this technique. RESULTS:Here we demonstrate a simple sample preparation method that could enhance the clarity o

SUBMITTER: Rai D 

PROVIDER: S-EPMC5956817 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature