G9a stimulates CRC growth by inducing p53 Lys373 dimethylation-dependent activation of Plk1.

ABSTRACT: Rationale: G9a is genetically deregulated in various tumor types and is important for cell proliferation; however, the mechanism underlying G9a-induced carcinogenesis, especially in colorectal cancer (CRC), is unclear. Here, we investigated if G9a exerts oncogenic effects in CRC by increasing polo-like kinase 1 (Plk1) expression. Thus, we further characterized the detailed molecular mechanisms. Methods: The role of Plk1 in G9a aberrant CRC was determined by performing different in vitro and in vivo assays, including assessment of cell growth by performing cell viability assay and assessment of signaling transduction profiles by performing immunoblotting, in the cases of pharmacological inhibition or short RNA interference-mediated suppression of G9a. Detailed mo

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5957015 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature