Transforming growth factor-β modulates pancreatic cancer associated fibroblasts cell shape, stiffness and invasion.

ABSTRACT:

Background

Tumor microenvironment consists of the extracellular matrix (ECM), stromal cells, such as fibroblasts (FBs) and cancer associated fibroblasts (CAFs), and a myriad of soluble factors. In many tumor types, including pancreatic tumors, the interplay between stromal cells and the other tumor microenvironment components leads to desmoplasia, a cancer-specific type of fibrosis that hinders treatment. Transforming growth factor beta (TGF-β) and CAFs are thought to play a crucial role in this tumor desmoplastic reaction, although the involved mechanisms are unknown.

Methods

Optical/fluorescence microscopy, atomic force microscopy, image processing techniques, invasion assay in 3D collagen I gels and real-time PCR were employed to investigate the effect of TGF-β on normal

SUBMITTER: Stylianou A 

PROVIDER: S-EPMC5957271 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature