Cell cycle pathway dysregulation in human keratinocytes during chronic exposure to low arsenite.

ABSTRACT:

Background

Arsenic is naturally prevalent in the earth's crust and widely distributed in air and water. Chronic low arsenic exposure is associated with several cancers in vivo, including skin cancer, and with transformation in vitro of cell lines including immortalized human keratinocytes (HaCaT). Arsenic also is associated with cell cycle dysregulation at different exposure levels in multiple cell lines. In this work, we analyzed gene expression in HaCaT cells to gain an understanding of gene expression changes contributing to transformation at an early time point.

Methods

HaCaT cells were exposed to 0 or 100nM NaAsO₂ for 7weeks. Total RNA was purified and analyzed by microarray hybridization. Differential expression with fold change≥|1.5| and p-value≤0.05 was de

SUBMITTER: Al-Eryani L 

PROVIDER: S-EPMC5957280 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature