PTRA - A reporter system for monitoring the intracellular dynamics of gene expression.

ABSTRACT: The presence of standardised tools and methods to measure and represent accurately biological parts and functions is a prerequisite for successful metabolic engineering and crucial to understand and predict the behaviour of synthetic genetic circuits. Many synthetic gene networks are based on transcriptional circuits, thus information on transcriptional and translational activity is important for understanding and fine-tuning the synthetic function. To this end, we have developed a toolkit to analyse systematically the transcriptional and translational activity of a specific synthetic part in vivo. It is based on the plasmid pTRA and allows the assignment of specific transcriptional and translational outputs to the gene(s) of interest (GOI) and to compare different genetic setups. By this,

SUBMITTER: Wagner SG 

PROVIDER: S-EPMC5957375 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature