Dynamics and control of sister kinetochore behavior during the meiotic divisions in Drosophila spermatocytes.

ABSTRACT: Sister kinetochores are connected to the same spindle pole during meiosis I and to opposite poles during meiosis II. The molecular mechanisms controlling the distinct behavior of sister kinetochores during the two meiotic divisions are poorly understood. To study kinetochore behavior during meiosis, we have optimized time lapse imaging with Drosophila spermatocytes, enabling kinetochore tracking with high temporal and spatial resolution through both meiotic divisions. The correct bipolar orientation of chromosomes within the spindle proceeds rapidly during both divisions. Stable bi-orientation of the last chromosome is achieved within ten minutes after the onset of kinetochore-microtubule interactions. Our analyses of mnm and tef mutants, where univalents instead of bivalents are present d

SUBMITTER: Chaurasia S 

PROVIDER: S-EPMC5957430 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature