Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells.

ABSTRACT:

Purpose

Corneal endothelial cells (CECs) are critical in maintaining clarity of the cornea. This study was initiated to develop peripheral blood mononuclear cell (PBMC)-originated, induced pluripotent stem cell (iPSC)-derived CECs.

Methods

We isolated PBMCs and programmed the mononuclear cells to generate iPSCs, which were differentiated to CECs through the neural crest cells (NCCs). The morphology of differentiating iPSCs was examined at regular intervals by phase contrast microscopy. In parallel, the expression of pluripotent and corneal endothelium (CE)-associated markers was investigated by quantitative real-time PCR (qRT-PCR). The molecular architecture of the iPSC-derived CECs and human corneal endothelium (hCE) was examined by mass spectrometry-based proteome sequenci

SUBMITTER: Ali M 

PROVIDER: S-EPMC5957521 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature