Overexpression of chloride channel-3 predicts unfavorable prognosis and promotes cellular invasion in gastric cancer.

ABSTRACT:

Background

Chloride channel-3 (CLC-3) has been reported to promote the proliferation and invasion in various tumors, yet little is known about its role in gastric cancer. In the present study, we investigated the clinical significance of CLC-3 and its biological role in gastric cancer.

Methods

Bioinformatic analysis, immunohistochemical staining, quantitative real-time polymerase chain reaction and Western blot assay were used to assess the expression of CLC-3 and its clinical significance in gastric cancer. The biological role of CLC-3 and its underlying mechanism were detected through in vitro experiments.

Results

CLC-3 was highly expressed in gastric cancer tissues and cell lines, and high levels of CLC-3 were significantly associated with adverse clinicopathologic

SUBMITTER: Peng J 

PROVIDER: S-EPMC5958948 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature