Combinatorial programming of human neuronal progenitors using magnetically-guided stoichiometric mRNA delivery.

ABSTRACT: Identification of optimal transcription factor expression patterns to direct cellular differentiation along a desired pathway presents significant challenges. We demonstrate massively combinatorial screening of temporally-varying mRNA transcription factors to direct differentiation of neural progenitor cells using a dynamically-reconfigurable magnetically-guided spotting technology for localizing mRNA, enabling experiments on millimetre size spots. In addition, we present a time-interleaved delivery method that dramatically reduces fluctuations in the delivered transcription factor copy numbers per cell. We screened combinatorial and temporal delivery of a pool of midbrain-specific transcription factors to augment the generation of dopaminergic neurons. We show that the combinatorial deliv

SUBMITTER: Azimi SM 

PROVIDER: S-EPMC5959718 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature