Optical detection of three modes of endocytosis at hippocampal synapses.

ABSTRACT: Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes. In this setting, we could distinguish three components of retrieval operating at ultrafast (~150-250 ms, ~20% of events), fast (~5-12 s, ~40% of events) and ultraslow speeds (>20 s, ~40% of events). While increasing Ca²⁺ slowed the fast events, increasing temperatu

SUBMITTER: Chanaday NL 

PROVIDER: S-EPMC5959719 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature