SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma.

ABSTRACT:

Background

Brahma (BRM) is the only catalytic subunit of the SWI/SNF chromatin-remodeling complex of Drosophila melanogaster. The function of SWI/SNF in transcription has long been attributed to its ability to remodel nucleosomes, which requires the ATPase activity of BRM. However, recent studies have provided evidence for a non-catalytic function of BRM in the transcriptional regulation of a few specific genes.

Results

Here we have used RNA-seq and ChIP-seq to identify the BRM target genes in S2 cells, and we have used a catalytically inactive BRM mutant (K804R) that is unable to hydrolyze ATP to investigate the magnitude of the non-catalytic function of BRM in transcription regulation. We show that 49% of the BRM target genes in S2 cells are regulated through mechanisms th

SUBMITTER: Jordan-Pla A 

PROVIDER: S-EPMC5960078 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature