Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca²⁺ microscopy.

ABSTRACT:

Objective

Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. However, current modalities for drug testing, such as electrophysiology and fluorescence imaging have inherent drawbacks. To circumvent these problems, we report the development of a bioluminescent Ca²⁺ indicator GmNL(Ca²⁺), and its application in a customized microscope for high-throughput drug screening.

Results

GmNL(Ca²⁺) gives a 140% signal change with Ca²⁺, and can image drug-induced changes of Ca²⁺ dynamics in cultured cells. Since bioluminescence requires application of a chemical substrate, which is consumed over ~ 30 min we made a dedicated microscope with automated drug

SUBMITTER: Suzuki K 

PROVIDER: S-EPMC5960208 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature