Distinct gating mechanism of SOC channel involving STIM-Orai coupling and an intramolecular interaction of Orai in Caenorhabditis elegans.

ABSTRACT: Store-operated calcium entry (SOCE), an important mechanism of Ca²⁺ signaling in a wide range of cell types, is mediated by stromal interaction molecule (STIM), which senses the depletion of endoplasmic reticulum Ca²⁺ stores and binds and activates Orai channels in the plasma membrane. This inside-out mechanism of Ca²⁺ signaling raises an interesting question about the evolution of SOCE: How did these two proteins existing in different cellular compartments evolve to interact with each other? We investigated the gating mechanism of Caenorhabditis elegans Orai channels. Our analysis revealed a mechanism of Orai gating by STIM binding to the intracellular 2-3 loop of Orai in C. elegans that is radically different from Orai gating by STIM binding

SUBMITTER: Kim KM 

PROVIDER: S-EPMC5960282 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature