Identification of Genetically Intact HIV-1 Proviruses in Specific CD4⁺ T Cells from Effectively Treated Participants.

ABSTRACT: Latent replication-competent HIV-1 persists in individuals on long-term antiretroviral therapy (ART). We developed the Full-Length Individual Proviral Sequencing (FLIPS) assay to determine the distribution of latent replication-competent HIV-1 within memory CD4⁺ T cell subsets in six individuals on long-term ART. FLIPS is an efficient, high-throughput assay that amplifies and sequences near full-length (∼9 kb) HIV-1 proviral genomes and determines potential replication competency through genetic characterization. FLIPS provides a genome-scale perspective that addresses the limitations of other methods that also genetically characterize the latent reservoir. Using FLIPS, we identified 5% of proviruses as intact and potentially replication competent. Intact proviruses were unequal

SUBMITTER: Hiener B 

PROVIDER: S-EPMC5960642 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature