ABO Blood Group Genotyping by Real-time PCR in Kazakh Population.

ABSTRACT:

Introduction

ABO blood group genotyping is a new technology in hematology that helps prevent adverse transfusion reactions in patients. Identification of antigens on the surface of red blood cells is based on serology; however, genotyping employs a different strategy and is aimed directly at genes that determine the surface proteins. ABO blood group genotyping by real-time PCR has several crucial advantages over other PCR-based techniques, such as high rapidity and reliability of analysis. The purpose of this study was to examine nucleotide substitutions differences by blood types using a PCR-based method on Kazakh blood donors.

Methods

The study was approved by the Ethics Committee of the National Center for Biotechnology. Venous blood samples from 369 healthy Kazakh blood

SUBMITTER: Tarlykov P 

PROVIDER: S-EPMC5960945 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature